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Journal of Lipid Research, Vol. 6, 7-9, January 1965
Copyright © 1965 by Lipid Research, Inc.

Isotope effects in gas-liquid chromatography of steroids

Marvin A. Kirschner and Mortimer B. Lipsett

Endocrinology Branch, National Cancer Institute, Bethesda, Maryland

When gas-liquid chromatography was performed with steroids containing H³ and C¹⁴, the H³/C¹⁴ ratio of the effluent was higher at the beginning and at the tail of the steroid peak. The H³/C¹⁴ ratio of the entire peak agreed with that determined by paper and thin-layer chromatography. Thus, gas-liquid chromatography on polar and nonpolar phases commonly used for chromatography of steroids apparently has sufficient resolving power to partially separate isotopic species whose molecular weights differ by 2% or less.

Supplementary key words gas-liquid chromatography • isotope separations • isotope ratios • steroids • doubly labeled steroid derivatives • isotopic species • resolving power

Submitted on May 11, 1964
Accepted on August 31, 1964

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