HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Whyte, M. Articles by Karmen, A. Search for Related Content PubMed Articles by Whyte, M. Articles by Karmen, A. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 6, 233-240, April 1965
Copyright © 1965 by Lipid Research, Inc.

Fatty acid esterification and chylomicron formation during fat absorption in rat: III. Positional relations in triglycerides and lecithin

Malcolm Whyte , Dewitt S. Goodman , and Arthur Karmen

Laboratory of Metabolism and Laboratory of Technical Development, National Heart Institute, Bethesda, Maryland

Chylomicron triglyceride and lecithin obtained after feeding mixtures of three or four free fatty acids to rats were hydrolyzed using pancreatic lipase and phospholipase A respectively. The distribution of fatty acid mass and radioactivity in the substrate materials and in the cleavage products was determined by gas-liquid chromatography.

The incorporation of exogenous (labeled) fatty acids into different positions was nearly random in the triglycerides but markedly nonrandom in lecithin where saturated acids, especially stearic acid, were predominantly esterified at the '-position and polyunsaturated fatty acids at the ßbeta;-position.

Specific radioactivity measurements were interpreted as showing greater than random amounts of endogenous (unlabeled) palmitic acid on the '-position of lecithin and, to a small extent, of endogenous linoleic acid on the ßbeta;-position of triglyceride.

Supplementary key words chylomicron • fatty acids • exogenous (C¹⁴) • endogenous • incorporation • triglyceride • lecithin • positional relations • rats

Submitted on July 31, 1964
Accepted on January 4, 1965

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. Zhou and A. Nilsson Sources of eicosanoid precursor fatty acid pools in tissues J. Lipid Res., October 1, 2001; 42(10): 1521 - 1542. [Abstract] [Full Text] [PDF]