Journal of Lipid Research, Vol. 6, 241-246, April 1965
Copyright © 1965 by Lipid Research, Inc.
Particle size and composition of dog lymph chylomicrons
Akira Yokoyama and D. B. Zilversmit
Department of Physiology, University of Tennessee Medical Units, Memphis, Tennessee
Chyle from the thoracic duct of dogs fed cream or corn oil was fractionated into chylomicrons of different particle size: <140 mµ, 140-200 mµ, and >200 mµ. The composition of chylomicrons was studied before and after washing with saline solutions. In general the percentages of protein, phospholipid, cholesterol, and cholesterol ester were greater in the small chylomicrons than in the larger ones.
Washing the chylomicrons did not appear to affect their composition. Chylomicrons collected at different times during fat feeding showed significant differences in phospholipid and cholesterol concentrations. Storage of lymph at 4° was accompanied by alterations in phospholipid and cholesterol concentrations of the chylomicrons. The composition of chylomicrons is consistent with the hypothesis that at some stage during their formation the nontriglyceride components are present on the surface of the oil droplet.
Supplementary key words dog • lymph • chylomicrons • particle size • composition • triglyceride • phospholipid • cholestorol • protein • gradient centrifugation
Submitted on August 28, 1964
Accepted on October 15, 1964
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