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Journal of Lipid Research, Vol. 6, 320-322, April 1965
Copyright © 1965 by Lipid Research, Inc.

Purification of phospholipids recovered from thin-layer chromatograms for infrared spectral analysis

A. H. Duthie and Stuart Patton

Department of Dairy Science, Pennsylvania State University, University Park, Pennsylvania

An application of the Folch washing procedure for the removal of contaminants from phospholipid classes recovered from thin-layer chromatograms is described. The infrared spectra from the purified phospholipids are satisfactory for identification. The pattern of the major milk phospholipids separated on a thin-layer plate is presented.

Supplementary key words thin-layer chromatogram • recovered phospholipids • purification • Folch wash • infrared spectra • milk

Submitted on September 2, 1964
Accepted on December 14, 1964

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