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Journal of Lipid Research, Vol. 6, 322-324, April 1965
Copyright © 1965 by Lipid Research, Inc.

Acetic anhydride-trifluoroacetic acid acetolysis for the estimation of glycerol in phosphatidyl choline by gas-liquid chromatography

K. S. Holla and David G. Cornwell

Department of Physiological Chemistry, The Ohio State University, Columbus, Ohio

Low glycerol recoveries are obtained when acetic anhydride-acetic acid acetolysis is used to dephosphorylate lecithin prior to glycerol (and fatty acid) analysis by saponification-acetylation and gas-liquid chromatography.

Quantitative glycerol recoveries are obtained for both phosphatidyl choline and phosphatidyl ethanolamine when a modified acetolysis using acetic anhydride-trifluoroacetic acid is employed. Modified acetolysis is limited to the analysis of glycerol alone since unsaturated fatty acids are destroyed.

Supplementary key words glycerol estimation • phosphatidyl choline • phosphatidyl ethanolamine • acetolysis • trifluoroacetic acid • glyceryl triacetate • gas-liquid chromatography

Submitted on August 18, 1964
Accepted on November 12, 1964

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