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Journal of Lipid Research, Vol. 6, 335-340, July 1965
Copyright © 1965 by Lipid Research, Inc.

Post-heparin phospholipase and fatty acid transesterification in human plasma

William C. Vogel , Will G. Ryan , J. L. Koppel , and J. H. Olwin

Coagulation Research Laboratory, Department of Surgery, Presbyterian-St. Luke's Hospital affiliated with the College of Medicine, University of Illinois, Chicago, Illinois

The incubation of post-heparin plasma and egg phosphatidyl ethanolamine in the presence of methanol, ethanol, or glycerol resulted in the synthesis of fatty acid esters of these alcohols. This synthesis requires fatty acids derived from the degradation of phosphatidyl ethanolamine. The fatty acid transesterification activity and the post-heparin phospholipase activity were found to involve the fatty acid at the 1-position of egg phosphatidyl ethanolamine.

Supplementary key words post-heparin plasma • man • phospholipase • transesterification • phosphatidyl ethanolamine • -fatty acid transferase • monoglyceride formation

Submitted on July 30, 1964
Accepted on January 18, 1965

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