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Journal of Lipid Research, Vol. 6, 383-389, July 1965
Copyright © 1965 by Lipid Research, Inc.

Radiohomogeneity of H³- and C¹⁴-labeled linoleic acid in vivo

Demetrios Sgoutas , Moon Ja Kim , and Fred A. Kummerow

The Burnsides Research Laboratory, University of Illinois, Urbana, Illinois

A mixture of linoleic acid-1-C¹⁴ and linoleic acid labeled with H³ in the chain was administered to rats, and the H³/C¹⁴ ratio in the linoleic acid recovered from liver and adipose tissue of the rat was compared with that in the administered mixture. Agreement in the H³/C¹⁴ ratios was taken as evidence that the H³-labeled linoleic acid was biologically indistinguishable from the C¹⁴-labeled.

These findings establish the in vivo stability of the H³ label in the fatty acid chain and the inability of the rat to discriminate between fatty acids labeled with H³ in the side chain and with C¹⁴ in the carboxyl group.

Supplementary key words linoleic acid-9,10,12,13-H³ • linoleic acid-1-C¹⁴ • H³/C¹⁴ isotope ratio • double-label isotope ratio method • rat • liver • lipids • adipose tissue • radio-homogeneity • stability of H³

Submitted on February 10, 1965
Accepted on April 5, 1965

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