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Journal of Lipid Research, Vol. 6, 431-433, July 1965
Copyright © 1965 by Lipid Research, Inc.

Colorimetric ultramicro method for the determination of free fatty acids

Milan Novák

Institute for Care of Mother and Child, Prague, Czechoslovakia

Previously described colorimetric ultramicro methods for the determination of serum and tissue fatty acids have been improved in sensitivity and selectivity by extracting cobalt rather than copper soaps by means of a solvent lighter than water instead of chloroform. The complex of Co++ with agr-nitroso-ßbeta;-naphthol is measured at 500 mµ.

Supplementary key words free fatty acids • extraction • colorimetric determination • cobalt soaps • serum

Submitted on May 8, 1964
Accepted on January 26, 1965


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