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Journal of Lipid Research, Vol. 6, 438-441, July 1965
Copyright © 1965 by Lipid Research, Inc.

Separation of cholesterol from its companions, cholestanol and ⁷-cholestenol, by thin-layer chromatography

A. Stewart Truswell and W. Derek Mitchell

Medical Research Council Atheroma Research Unit, Western Infirmary, Glasgow, Scotland

A two-step thin-layer chromatographic procedure is described for separation of cholesterol from its naturally occurring companions. Impregnation of Silica Gel G with silver nitrate retards cholesterol relative to cholestan-3ßbeta;-ol and ⁷-cholestenol, which can be separated from each other on a second plate by reversed-phase thin-layer chromatography at low temperature.

Supplementary key words cholesterol • cholestanol • ⁷-cholestenol • separation • thin-layer chromatography • silver nitrate • interference Liebermann-Burchard estimation • ethanolic ammonia extraction

Submitted on May 12, 1964
Accepted on March 11, 1965

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