HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Parker, F. Articles by Peterson, N. F. Search for Related Content PubMed Articles by Parker, F. Articles by Peterson, N. F. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 6, 455-460, October 1965
Copyright © 1965 by Lipid Research, Inc.

Quantitative analysis of phospholipids and phospholipid fatty acids from silica gel thin-layer chromatograms

Frank Parker and Norman F. Peterson

Division of Dermatology, Department of Medicine, University of Washington School of Medicine, Seattle, Washington

An improved procedure for the quantitative assay of phospholipids separated by TLC is described, in which a specially washed Silica Gel H and a newly designed chromatography unit are employed.

Analysis of phospholipid phosphorus and phospholipid fatty acids was accomplished in the presence of silica gel scraped directly from the chromatoplates. Recovery of phosphorus ranged from 96 to 100%.

The washing of the Silica Gel H with chloroform-methanol-formic acid resulted in extremely low phosphorus blanks on the silica gel, and also removed impurities which were found to cause various peaks on GLC.

Supplementary key words thin-layer chromatography • phospholipids • phosphorus determination • fatty acid composition • saturation chamber • impurities • Silica Gel H • 2',7'-dichlorofluorescein

Submitted on April 15, 1965
Accepted on June 25, 1965

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				P. Tso, K. Ding, S. DeMichele, and Y.-S. Huang Intestinal Absorption and Lymphatic Transport of a High {gamma}-Linolenic Acid Canola Oil in Lymph Fistula Sprague-Dawley Rats J. Nutr., February 1, 2002; 132(2): 218 - 221. [Abstract] [Full Text] [PDF]

				P. Tso, T. Lee, and S. J. Demichele Lymphatic absorption of structured triglycerides vs. physical mix in a rat model of fat malabsorption Am J Physiol Gastrointest Liver Physiol, August 1, 1999; 277(2): G333 - G340. [Abstract] [Full Text] [PDF]