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Journal of Lipid Research, Vol. 6, 461-465, October 1965
Copyright © 1965 by Lipid Research, Inc.

Purity of cholesterol to be used as a primary standard

J. H. Williams , M. Kuchmak , and R. F. Witter

U.S. Department of Health, Education, and Welfare, Public Health Service, Communicable Disease Center, Heart Disease Control Unit, Lipid Standardization Laboratory, Atlanta, Georgia

Cholesterol preparations from 14 commercial sources were purified by the dibromide method. The cholesterol contents of the original and purified preparations were determined by gas-liquid chromatography. Calculation of the results of the gas chromatographic analyses from the areas of the peaks rather than from their heights gave more precise results. Purified samples had higher contents of cholesterol, higher melting points, and narrower melting point ranges, and exhibited fewer contaminants on thin-layer chromatoplates than did the original preparations.

Supplementary key words cholesterol • primary standard • purification • cholesterol dibromide • quantitative gas-liquid chromatography • thin-layer chromatography

Submitted on March 2, 1965
Accepted on June 2, 1965

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