Journal of Lipid Research, Vol. 6, 512-517, October 1965
Copyright © 1965 by Lipid Research, Inc.
New method for partial delipidization of serum lipoproteins
Anders Gustafson
Cardiovascular Section, Oklahoma Medical Research Institute, Oklahoma City, Oklahoma
A new method of partial delipidization of serum lipoproteins has been developed which removes neutral lipids preferentially and yields phospholipid-protein residues in soluble form without the use of detergents. Purified serum lipoprotein fractions were lyophilized in the presence of insoluble starch, which protected the proteins against damage during freezing, and the dry lipoproteins were partially delipidized by consecutive extractions with n-heptane. All neutral lipids and some phospholipids, mainly lecithin, were removed. Soluble phospholipid-protein residues were recovered by extraction with buffer solutions in yields ranging from 50 to 90%.
The characteristic differences in the phospholipid-protein ratios between individual lipoprotein fractions are probably determined by the binding properties, dependent on primary structure, of the corresponding protein moieties.
Supplementary key words lipoproteins • high-density • low-density • very low-density • human serum • partial delipidization • lyophilization • insoluble starch • n-heptane • neutral lipids • phospholipids
Submitted on March 4, 1965
Accepted on June 28, 1965
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