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Journal of Lipid Research, Vol. 6, 572-574, October 1965
Copyright © 1965 by Lipid Research, Inc.

Purification of neutral lipid fractions by thin-layer chromatography on aluminum oxide

J. M. Lederkremer and Ralph M. Johnson

Institute of Nutrition and Food Technology and the Department of Physiological Chemistry, The Ohio State University, Columbus, Ohio

Two-stage thin-layer chromatography on Aluminum Oxide G has been used to separate triglycerides, cholesterol, cholesterol esters, and diglycerides from contaminating fatty acids present in the lipid mixtures. Negligible hydrolysis of glycerol esters occurs. The method is particularly useful in metabolic experiments conducted in vitro, in which radioactive fatty acids are employed as precursors.

Supplementary key words two-stage thin-layer chromatography • Aluminum Oxide G • separation • neutral lipids • triglycerides • cholesterol esters • glycerides • free fatty acids

Submitted on February 24, 1965
Accepted on May 24, 1965

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