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Journal of Lipid Research, Vol. 7, 146-149, January 1966
Copyright © 1966 by Lipid Research, Inc.

Use of a filter-paper disk assay in the measurement of lipid biosynthesis

Howard Goldfine

Department of Bacteriology and Immunology, Harvard Medical School, Boston, Massachusetts

The precipitation by trichloroacetic acid (TCA) of radioactive lipids on disks of filter-paper, followed by extraction with TCA and water to remove soluble radioactive precursors, provides the means for a convenient assay for lipid biosynthesis. Large numbers of disks can be washed in the same vessel, dried, and assayed for radioactivity with liquid scintillation counting equipment. Extraction of disks with lipid solvents provides a convenient control for the possible presence of TCA- and water-insoluble, nonlipid products. The application of the method to the assay of cyclopropane fatty acid synthetase is shown.

Supplementary key words determination • radioactive • lipids • biosynthesis • precipitation • trichloroacetic acid • filter-paper disk • cyclopropane fatty acid synthetase

Submitted on August 9, 1965
Accepted on September 15, 1965


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