J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Journal of Lipid Research, Vol. 7, 167-169, January 1966
Copyright © 1966 by Lipid Research, Inc.

Modified enzymatic procedure for the routine determination of glycerol and triglycerides in plasma

Carmine J. Spinella and Milton Mager

Biochemistry Laboratory, U. S. Army Research Institute of Environmental Medicine, Natick, Massachusetts

A modification of Wieland's enzymatic procedure for glycerol analysis is presented. It is simple and precise, and readily applicable to the routine analysis of plasma and tissue glycerol and triglycerides. Optimal precision is obtained in samples containing 0.003-0.400 µmole of glycerol per ml, which in this method is equivalent to plasma levels of 0.011 to 1.40 µmoles/ml.

Supplementary key words determination • glycerol • triglycerides • plasma • enzymatic • modification • metaphosphoric acid • glycerokinase

Submitted on June 1, 1965
Accepted on August 23, 1965


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