Journal of Lipid Research, Vol. 7, 170-174, January 1966
Copyright © 1966 by Lipid Research, Inc.

Quantitative micro determination and isolation of plasmalogen aldehydes as 2,4-dinitrophenylhydrazones

Ira Katz and Mark Keeney

Department of Dairy Science, University of Maryland, College Park, Maryland

A micro spectrophotometric procedure for the quantitative determination of plasmalogen aldehydes is described which utilizes simultaneous methanolysis and formation of 2,4-dinitrophenylhydrazones. After isolation of the hydrazones by thin-layer chromatography, the aldehydes can be regenerated, reduced, acetylated, and then analyzed by gas-liquid chromatography. Identification of the plasmalogen aldehydes obtained from rumen holotrich protozoa is described.

Supplementary key words plasmalogen aldehydes • determination • 2,4-dinitrophenylhydrazones • spectrophotometry • thin-layer chromatography • aldehyde • regeneration • reduction • composition • gas-liquid chromatography • ethanolamine glycerophosphatides • rumen protozoa

Submitted on June 3, 1965
Accepted on September 15, 1965

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