Journal of Lipid Research, Vol. 7, 22-26, January 1966
Copyright © 1966 by Lipid Research, Inc.
Environmental temperature and choline requirements in rats. II: Choline and methionine requirements for lipotropic activity
J. S. Chahl and C. C. Kratzing
Department of Physiology, University of Queensland, Brisbane, Australia
Young rats were fed choline-deficient diets and maintained at different environmental temperatures. The hepatic lipid level remained normal in rats at 2° when 25 mg of choline per 100 g of food was fed; 50 mg of choline per 100 g food was required at 21° and 100 mg of choline per 100 g food at 33° to prevent excessive lipid accumulation. These values were equivalent to a mean daily intake per rat of 3 mg of choline at 2°, 5.5 mg at 21°, and 7 mg at 33° respectively.
When the growth rate was slower owing to a slight inadequacy of histidine in the basal choline-deficient diet, normal hepatic lipid was maintained by supplements of 50 mg of choline per 100 g food at 21° and 33°.
Increasing the methionine content of the diet two- or three-fold from a basal value of 340 mg per 100 g food was as effective as 200 mg of choline per 100 g of food in lowering hepatic lipids at 2°, 21°, and 33°.
Supplementary key words choline • deficiency • requirement • prevention • fatty liver • environmental temperature • methionine supplementation • rat
Submitted on February 4, 1965
Accepted on August 5, 1965
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