Journal of Lipid Research, Vol. 7, 65-72, January 1966
Copyright © 1966 by Lipid Research, Inc.

Particle-size distribution of very low density plasma lipoproteins during fat absorption in man

Edwin L. Bierman , Thomas L. Hayes , James N. Hawkins , Alicia M. Ewing , and Frank T. Lindgren

Department of Medicine, University of Washington School of Medicine and Veterans Administration Hospital, Seattle, Washington, and the Donner Laboratory, Lawrence Radiation Laboratory, University of California, Berkeley, California

The size distributions of electrophoretically isolated subfractions of the very low density human plasma lipoproteins have been determined using electron microscopy. The primary and secondary particles observed in plasma of normal subjects after fat ingestion appear to have similar size distributions. Particles produced by corn oil feeding can be fixed by the osmium tetroxide reaction while those produced by butter fat feeding could not be fixed or made visible by this technique. Good agreement between particle size as measured by electron microscopy and particle size as predicted by ultra-centrifugal analysis was obtained.

Supplementary key words absorption • fat • primary • secondary • particles • size • plasma • very low density lipoproteins • chylomicrons • electron microscopy • corn oil • butter fat • osmium fixation • ultracentrifugation • man

Submitted on July 15, 1965
Accepted on August 31, 1965

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