Journal of Lipid Research, Vol. 7, 95-102, January 1966
Copyright © 1966 by Lipid Research, Inc.
Specific sites of fatty acid and sterol synthesis in isolated skin components
S. C. Brooks , V. C. Godefroi , and W. L. Simpson
Detroit Institute of Cancer Research and Departments of Physiological Chemistry and Pathology, Wayne State University School of Medicine, Detroit, Michigan
Metabolic studies on isolated mouse skin components were undertaken to determine the specific sites of fatty acid and sterol synthesis. The concentrations of long-chain fatty acids and sterols and the incorporation of radioactivity from acetate-1-14C into these lipids are reported for various skin components and intact whole skin.
Only fatty acids having chain lengths of 18 carbons or less were produced by the connective tissue cells of the dermis, while fatty acids containing 20 carbons or more, as well as the acids of 18 carbons or less, were synthesized in the upper dermis (papillary reticulum). The upper dermis also produced significant quantities of eicosenoic acid and of an octadecadienoic acid (not linoleic acid), and incorporated labeled acetate into fatty acids containing an odd number of carbons.
Removal of the epidermis and adnexa diminished sterol synthesis. However, the upper region of the dermis was capable of synthesizing, from acetate, large quantities of unidentified nonsaponifiable lipids which were neither sterols nor squalene.
Supplementary key words metabolism • skin components • epidermis • dermis • mouse • biosynthesis • fatty acid • cholesterol • 
7-cholestenol • squalene • acetate-1-14C • gas-liquid chromatography • trimethylsilyl ethers
Submitted on February 25, 1965
Accepted on August 27, 1965
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