Journal of Lipid Research, Vol. 7, 204-209, March 1966
Copyright © 1966 by Lipid Research, Inc.

Incorporation of acetate-1-¹⁴C into lipids by the perfused liver of normal, X-irradiated, or partially hepatectomized rats

Guenter G. Bartsch and Georg B. Gerber

Physiologisch-Chemisches Institut der Universität, Cologne, Germany, and Euratom and Centre d'Etude de l'Energie Nucléaire, Department Radiobiology, Mol, Belgium

In order to study lipid metabolism in the liver without interference due to transport from and to the liver the isolated livers of normal, X-irradiated, and partially hepatectomized rats were perfused with acetate-1-¹⁴C and the distribution of radioactivity in total lipids, total fatty acids, individual lipids, and fatty acids of individual lipids was determined. In X-irradiated animals, an increased incorporation of acetate into many lipids, particularly into cholesterol, was observed. Lipids in the liver of the partially hepatectomized rats exhibited a marked increase in triglyceride content together with a decreased rate of incorporation into all but the phospholipid fractions.

It is concluded that the increase usually observed in lipid pontent of the regenerating liver is due to the changes in transcort rather than to changes in synthesis. The changes observed in irradiated liver could be the result of alterations in the metabolism of precursors common to most lipids.

Supplementary key words rat • normal • X-irradiated • partially hepatectomized • liver perfusion • incorporation • acetate-1-¹⁴C • total lipids • lipid fractions • fatty acids

Submitted on July 23, 1965
Accepted on October 25, 1965

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