Journal of Lipid Research, Vol. 7, 264-269, March 1966
Copyright © 1966 by Lipid Research, Inc.
Use of constant composition polyvinylpyrrolidone columns to study the interaction of fat particles with plasma
David D. O'Hara , Daniel Porte Jr. , and Robert H. Williams
Department of Medicine, University of Washington, Seattle, Washington
Fat particles (lipoproteins of Sf > 400) have been obtained from dog and human lymph and from human post-alimentary plasma. They were flocculated by a solution of 5% polyvinylpyrrolidone (PVP) in 10% NaCl and layered at the bottom of tubes of PVP of various concentrations between 2% and 5% (w/v). As the concentration of PVP increased, a greater proportion of the particles accumulated at the top of the tube ("primary" particles). When the concentration of PVP in the tube was held constant at 3% PVP, dilution of the sample with plasma was found to produce an increased proportion of bottom particles ("secondary" particles). This observation suggests that bottom particles result from an interaction of top particles with plasma.
Supplementary key words dog • man • lymph • plasma • primary • secondary • fat particles • interconversion • separation • polyvinylpyrrolidone column • constant composition • gradient • very low density lipoproteins
Submitted on August 25, 1965
Accepted on November 27, 1965
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