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Journal of Lipid Research, Vol. 7, 324-327, March 1966
Copyright © 1966 by Lipid Research, Inc.

Complete separation of lipid classes on a single thin-layer plate

C. P. Freeman and D. West

Unilever Research Laboratory, Colworth House, Sharnbrook, Bedford, England

A double-development procedure employing first a polar and then a nonpolar solvent system is described for the complete separation by thin-layer chromatography of the main lipid classes encountered in natural lipids. For better quantification, long plates (34 cm) are employed. Diglycerides were separated from cholesterol, 1,2- from 1,3-diglycerides, and monoglycerides from phospholipids.

Supplementary key words double-development • thin-layer chromatography • quantification • lipid classes

Submitted on August 17, 1965
Accepted on November 17, 1965

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