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Journal of Lipid Research, Vol. 7, 327-329, March 1966
Copyright © 1966 by Lipid Research, Inc.

Two-column gas-liquid chromatography of fatty acid methyl esters

Thomas M. Smith and Harold B. White Jr.

Department of Biochemistry, University of Mississippi School of Medicine, Jackson, Mississippi

Fatty acid methyl esters were separated into fractions according to chain length on a nonpolar gas-liquid chromatographic column. These fractions were collected and rechromatographed on a polar column. Temperature programming was used in both cases. Data are given for the accuracy of the double procedure applied to a synthetic mixture.

Supplementary key words two-stage • gas-liquid chromatography • fatty acid methyl esters • quantification • accuracy

Submitted on August 2, 1965
Accepted on October 25, 1965

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