Journal of Lipid Research, Vol. 7, 368-371, May 1966
Copyright © 1966 by Lipid Research, Inc.

Lipid composition of fat particles from normal man and patients with idiopathic hypertriglyceridemia

Daniel Porte Jr. , David D. O'Hara , and Robert H. Williams

Department of Medicine, University of Washington, Seattle, Washington

Lipemic plasma from normal and hyperlipemic patients has been fractionated on columns of 3% (w/v) aqueous polyvinylpyrrolidone (PVP) and the lipid composition of the separated fat particles (S_f > 400) measured. Plasma from patients with carbohydrate-induced lipemia on fat-free diets contained particles with a greater percentage of cholesterol and phospholipid than either normal primary particles (which float to the top of 3% PVP columns) or secondary particles (which remain concentrated just above the plasma layer at the bottom of the 3% PVP column). These "hyperlipemia" particles remained in the lower half of 3% PVP columns, which allowed easy separation from primary (top) particles. In the same hypertriglyceridemic patients primary (top) particles with the usual lipid composition were isolated from plasma 8 hr after ingestion of 200 g of corn oil, but 24 hr after the meal, primary (top) particles isolated in the same way contained a higher percentage of cholesterol than normal primary particles. High-cholesterol primary (top) particles were isolated from the patients mentioned and also from patients with fat-induced lipemia after an overnight fast on a regular fat-containing diet.

It is concluded that the lipid composition of primary particles is variable and reflects the length of time these particles have been in the general circulation.

Supplementary key words plasma • fat particle • lipid composition • hyperlipemia • lipoprotein • polyvinylpyrrolidone • man • fat load

Submitted on November 12, 1965
Accepted on December 21, 1965

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