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Journal of Lipid Research, Vol. 7, 442-445, May 1966
Copyright © 1966 by Lipid Research, Inc.

Determination of fatty acid content and composition in ultramicro lipid samples by gas-liquid chromatography

Francis M. Archibald and Vladimir P. Skipski

Division of Experimental Chemotherapy, Sloan-Kettering Institute for Cancer Research, Rye, New York

Simplified quantitative manipulations of very small amounts (30 µg) of lipids for determination of fatty acid content and composition by gas-liquid chromatography after (a) methanolysis (b) reduction and acetylation are described.

Supplementary key words determination • ultramicro • fatty acids • methyl esters • alcohol acetates • glycerol • gas-liquid chromatography • liver lipids

Submitted on August 20, 1965
Accepted on January 13, 1966

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