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Journal of Lipid Research, Vol. 7, 445-447, May 1966
Copyright © 1966 by Lipid Research, Inc.

Preparation of phosphatidyl inositol from bakers' yeast

William E. Trevelyan

Distillers Company Ltd., Great Burgh, Epsom, Surrey, England

Phosphatidyl inositol and a cardiolipin-like phospholipid survive the autolysis which is induced in yeast by treating it with toluene, whereas other phospholipids are extensively degraded. Lipid from autolyzed yeast was suspended in isopropanol to extract lecithin and neutral lipid. The insoluble phosphatidyl inositol and cardiolipin were then separated by chromatography on silicic acid.

Supplementary key words yeast • autolysis • toluene • phosphatidyl inositol • cardiolipin

Submitted on October 29, 1965
Accepted on January 20, 1966


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