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Journal of Lipid Research, Vol. 7, 544-550, July 1966
Copyright © 1966 by Lipid Research, Inc.

Two-dimensional chromatography on silica gel-loaded paper for the microanalysis of polar lipids

R. E. Wuthier

Forsyth Dental Center, Harvard School of Dental Medicine, Boston, Massachusetts

Two-dimensional chromatography on commercially available silica gel-loaded paper for the microanalysis of polar lipids from various tissues is described. All common phospholipids and their lyso derivatives can be reproducibly separated. As many as 22 lipid components were separated on a single chromatogram. Improved methods for staining lipids and for determining phosphorus in the chromatographic spots are reported.

Supplementary key words paper chromatography • two-dimensional • silica gel-loaded paper • phospholipids • P determination • polar • lipids • cartilage • bone • Rhodamine 6G staining • Dragendorff stain

Submitted on October 12, 1965
Accepted on April 18, 1966


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