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Journal of Lipid Research, Vol. 7, 551-557, July 1966
Copyright © 1966 by Lipid Research, Inc.

Thin-layer electrophoresis of serum lipoproteins

Pentti K. Reissell , Lillian M. Hagopian , and Frederick T. Hatch

Arteriosclerosis Unit, Medical Services, Massachusetts General Hospital and Department of Medicine, Harvard Medical School, Boston, Massachusetts

A method is described for semiquantitative study of serum lipoproteins, with particular reference to the very low density fractions involved in transport of triglycerides. Serum samples were subjected to electrophoresis in thin layers of starch granules; lipids were extracted from starch segments and subjected to thin-layer chromatography; lipid fractions were recovered from the adsorbent and quantified colorimetrically.

The procedure is applicable to the study of alimentary and endogenous hyperlipidemias in man, and to radioactive tracer experiments. Over-all recoveries of lipids were variable and averaged 81% for cholesterol and 76% for triglycerides.

Supplementary key words serum • lipoproteins • thin-layer electrophoresis • starch granule layer • very low density lipoproteins • lymph • alimentary lipemia

Submitted on December 8, 1965
Accepted on April 6, 1966

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