J. Lipid Res.
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Journal of Lipid Research, Vol. 7, 561-564, July 1966
Copyright © 1966 by Lipid Research, Inc.

Protein content and osmotic behavior of isolated fat cells

John J. Lech and Deane N. Calvert

Department of Pharmacology, Marquette University School of Medicine, Milwaukee, Wisconsin

Fat cells isolated by a modification of the method of Rodbell were washed in protein-free buffers, which allowed determination of their protein content. Osmotic behavior and response to norepinephrine and insulin were unimpaired.

Supplementary key words isolated fat cells • protein determination • osmotic properties • response • norepinephrine • insulin

Submitted on October 25, 1965
Accepted on March 23, 1966


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