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Journal of Lipid Research, Vol. 7, 570-574, July 1966
Copyright © 1966 by Lipid Research, Inc.

Rapid quantitative measurement of lung tissue phospholipids

Louis Gluck , Marie V. Kulovich , and Spencer J. Brody

Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut

A rapid procedure for the separation of phospholipids of lung tissue into acidic and nonacidic fractions by means of diethylaminoethyl cellulose acetate microcolumns is described. The fractions are then resolved into individual phospholipids by thin-layer chromatography and quantified by transmission densitometry.

Supplementary key words lung • phospholipids • acidic • non-acidic • DEAE cellulose columns • thin-layer chromatography • quantification • charring • transmission densitometry

Submitted on January 22, 1966
Accepted on March 23, 1966

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