Journal of Lipid Research, Vol. 7, 627-633, September 1966
Copyright © 1966 by Lipid Research, Inc.

Purification of serologically active phosphoinositides of Mycobacterium tuberculosis

Mary C. Pangborn and John A. McKinney

Division of Laboratories and Research, New York State Department of Health, Albany, New York

Glycolipids extracted with pyridine from three strains of Mycobacterium tuberculosis were fractionated. Phosphatidyl inositol, two phosphatidyl inositol dimannosides (A and M), and two phosphatidyl inositol pentamannosides (G and K) were separated and purified by a combination of solvent fractionations and chromatography on silicic acid. In each of these lipids, palmitic and tuberculostearic acids accounted for more than 90% of the total fatty acids. Mole ratios of fatty acid to P were: for phosphatidyl inositol, 2; for G and M, 3; for A and K, 4. Certain mixtures of A or M with G fixed complement with human sera from cases of tuberculosis. Two serologically inactive dimannosides, each containing two fatty acid ester groups per atom of P, were also present.

Supplementary key words Mycobacterium tuberculosis • phosphatidyl inositol • di- and pentamannosides • phosphoinositides • complement fixation • glycolipids • tuberculosis

Submitted on January 26, 1966
Accepted on April 11, 1966

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