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Journal of Lipid Research, Vol. 7, 649-656, September 1966
Copyright © 1966 by Lipid Research, Inc.

Release of free fatty acids from Ehrlich ascites tumor cells

Arthur A. Spector and Daniel Steinberg

Laboratory of Metabolism, National Heart Institute, National Institutes of Health, Bethesda, Maryland

Ehrlich ascites tumor cells release free fatty acids (FFA) during in vitro incubation in media that contain albumin. The released FFA are derived by lipolysis from endogenous lipid esters. Addition of glucose to the incubation medium greatly decreases the quantity of fatty acid released by the cells. Cyanide, which inhibits endogenous lipid oxidation but not lipolysis, increases the quantity of fatty acid released to media containing albumin and causes free fatty acid to accumulate in the cells in the absence of exogenous albumin. The release of fatty acid, either preformed or derived by lipolysis during prolonged incubations, occurs under conditions of net fatty acid uptake from the incubation medium. Net release of fatty acid from the cell occurs only when fatty acid-extracted albumin is present in the extracellular medium; extrapolation of the data suggests that net release will not occur under physiological conditions.

It is postulated that free fatty acid uptake and release are independent processes, the direction of net fatty acid movement being determined by the relationship between cellular free fatty acid concentration (regulating efflux) and the molar ratio of free fatty acid to albumin in the extracellular medium (regulating uptake).

Supplementary key words free fatty acid • transport • release • efflux • uptake • lipid utilization • oxidation • esters • palmitate • albumin • glucose • cyanide • Ehrlich ascites tumor

Submitted on February 15, 1966
Accepted on May 18, 1966


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