Journal of Lipid Research, Vol. 7, 671-677, September 1966
Copyright © 1966 by Lipid Research, Inc.
Fatty acid synthesis in cell-free system from rabbit aorta
Arthur F. Whereat
Departments of Medicine and Biochemistry, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania
The objectives of this study were to identify the subcellular fraction responsible for fatty acid synthesis in rabbit aorta and to determine the effect of cholesterol feeding on the system. A method for homogenization of aorta is described which permitted the isolation of subcellular components of aorta, including mitochondria that were morphologically and functionally intact. Mitochondria were identified as the major site of fatty acid synthesis in this tissue. Cofactor requirements and products showed that the synthetic system operates by chain elongation.
Mitochondria from atherosclerotic aortas incorporated acetate into fatty acids faster than did mitochondria from control aortas. It is concluded that cholesterol feeding leads to alterations of aortic mitochondrial function and accelerates the fatty acid elongation pathway.
Supplementary key words aorta • homogenization • mitochondria • electron microscopy • fatty acid • biosynthesis • chain elongation • cholesterol feeding • atherosclerosis • rabbit • oxidative phosphorylation • nonprotein stimulants • NADH • Schmidt degradation
Submitted on April 19, 1966
Accepted on June 2, 1966
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