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Journal of Lipid Research, Vol. 7, 678-683, September 1966
Copyright © 1966 by Lipid Research, Inc.

Enzymatic synthesis of cytidine diphosphate diglyceride

James R. Carter and Eugene P. Kennedy

Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts

Evidence is presented for the enzymatic formation of cytidine diphosphate diglyceride in microsomal preparations from guinea pig liver according to the reaction:

CTP + phosphatidic acid rlhar2 CDP-diglyceride + p—O—P.

Conditions have been found in which the incorporation of labeled CTP into CDP-diglyceride is almost entirely dependent upon added phosphatidic acid. The incorporation of CMP into lipid is very slight. A substantial net synthesis of CDP-diglyceride takes place under these conditions. Some properties of the enzyme system are described.

Supplementary key words cytidine nucleotide • CDP-diglyceride • enzymatic • biosynthesis • phosphatidic acid • microsomes • guinea pig • liver • dependence • physicochemical dispersion state

Submitted on May 3, 1966
Accepted on June 16, 1966


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