Journal of Lipid Research, Vol. 7, 739-744, November 1966
Copyright © 1966 by Lipid Research, Inc.

Biologically active metabolite of vitamin D₃ from bone, liver, and blood serum

Judith Lund and Hector F. Deluca

Department of Biochemistry, University of Wisconsin, Madison, Wisconsin

Radioactive metabolites present in bone, blood, liver, and feces of rats given ³H vitamin D₃ have been isolated. Of these the aqueous soluble metabolite(s) from tissue and all those isolated from feces did not cure rickets in rats, while all the others were at least partially active in this regard. One of the metabolites proved to be as active as the parent vitamin in curing rickets and was found in large amounts in liver, blood, and bone.

As much as 50-80% of the radioactivity in bone was found in this metabolite after a 500 IU oral dose of ³H vitamin D₃. With 10 IU doses of 1,2-³H vitamin D₃, most of the radioactivity of the organs examined was found in this metabolite fraction. This metabolite appears to be more polar than vitamin D and is not an esterified form of the vitamin nor a complex of the vitamin with tissue lipids. Its possible role as the metabolically active form of the vitamin is discussed.

Supplementary key words vitamin D₃ • metabolite(s) • biologically active • bone • liver • serum • low doses • rickets • rat • man

Submitted on May 13, 1966
Accepted on July 20, 1966

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