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Journal of Lipid Research, Vol. 7, 763-771, November 1966
Copyright © 1966 by Lipid Research, Inc.

Biosynthesis of cholestanol: 5agr-cholestan-3-one reductase of rat liver

Sarah Shefer , Susan Hauser , and Erwin H. Mosbach

Department of Laboratory Diagnosis, Public Health Research Institute of the City of New York, Inc., and the Bureau of Laboratories, New York City Department of Health, New York, N. Y.

The 3-bgr-hydroxysteroid dehydrogenase of rat liver which catalyzes the conversion of 5agr-cholestan-3-one to 5agr-cholestan-3bgr-ol is localized mainly in the microsomal fraction. The enzyme required NADPH as hydrogen donor and differed from the known 3-bgr-hydroxysteroid dehydrogenases of the C19 series in being inactive in the presence of NADH. The microsomal preparations did not reduce the 3-keto groups of cholest-4-en-3-one, cholest-5-en-3-one, or 5bgr-cholestan-3-one to the corresponding 3bgr-hydroxy compounds. The conversion of 5agr-cholestan-3-one to 5agr-cholestan-3bgr-ol was only slightly inhibited by the reaction product or by other monohydroxy steroids, but a strong inhibitory effect was noted with cholest-5-en-3-one, 5agr-cholestane-3bgr,7agr-diol and 5agr-cholestan-7-on-3bgr-ol.

The microsomes, but not high speed supernatant solution, catalyzed the reverse of the cholestanone reductase reaction, namely the conversion of 5agr-cholestan-3bgr-ol to 5agr-cholestan-3-one in the presence of oxygen and an NADP-generating system.

The action of the microsomal preparations upon 5agr-cholestan-3-one produced 5agr-cholestan-3agr-ol in addition to the 3bgr-epimer. The 3-agr-hydroxysteroid dehydrogenase involved functioned with either NADH or NADPH as hydrogen donor. The ratio of 5agr-cholestan-3bgr-ol to 5agr-cholestan-3agr-ol formed from 5agr-cholestan-3-one was approximately 10:1 and was independent of the sex of the animal from which the microsomes were prepared.

Supplementary key words 5agr-cholestan-3-one • cholestanone reductase • 5agr-cholestan-3bgr-ol • biosynthesis • 5agr-cholestan-3agr-ol • 3-bgr-hydroxysteroid dehydrogenase • 3-agr-hydroxysteroid dehydrogenase • microsomal enzyme • rat • liver

Submitted on April 13, 1966
Accepted on June 30, 1966


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