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Journal of Lipid Research, Vol. 7, 789-792, November 1966
Copyright © 1966 by Lipid Research, Inc.

Release of carbohydrates from sphingoglycolipid by osmium-catalyzed periodate oxidation followed by treatment with mild alkali

Sen-Itiroh Hakomori

Laboratory for Carbohydrate Research, Departments of Biological Chemistry and Medicine, Harvard Medical School and Massachusetts General Hospital, Boston, Massachusetts

The carbohydrate moiety of sphingoglycolipid, after preliminary acetylation, can be released by periodate oxidation catalyzed by a trace amount of osmium tetroxide, followed by alkaline treatment.

Cerebroside, lactosyl ceramide, hematoside, globoside, and gangliosides were degraded to yield, respectively, galactose, lactose, sialyl lactose, a tetrasaccharide, and various oligosaccharides containing sialic acid. Oligosaccharides were separated by paper chromatography and paper electrophoresis. The procedure is useful for characterizing micromolar amounts of sphingoglycolipids.

Supplementary key words osmium-catalyzed • periodate • oxidation • acetylated • sphingoglycolipid • cleavage • sphingosine-carbohydrate bond • separation • oligosaccharides

Submitted on May 19, 1966
Accepted on July 27, 1966


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