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Journal of Lipid Research, Vol. 8, 38-45, January 1967
Copyright © 1967 by Lipid Research, Inc.

Isolation and analysis of free fatty aldehydes from rat, dog, and bovine heart muscle

John R. Gilbertson , William J. Ferrell , and Rose A. Gelman

Department of Biochemistry and Nutrition, Graduate School of Public Health, University of Pittsburgh, Pennsylvania

Procedures are described for the isolation of free fatty aldehydes from total lipid extracts of rat, dog, and bovine heart muscle. These aldehydes did not arise from hydrolysis of the naturally occurring alkenyl ethers in either the extraction or isolation procedures.

The free fatty aldehydes were present in heart muscle in amounts between 0.27 and 0.56 µmole per 100 mg of lipid.

Hexadecanal and octadecanal are the principal free fatty aldehydes present, except in dog heart muscle where an unidentified, unusual aldehyde constitutes 20% of the mixture.

Supplementary key words free fatty aldehydes • rat • dog • bovine • heart muscle • thin-layer chromatography • p-nitrophenylhydrazones • dimethyl acetals • gas-liquid chromatography • silicic acid column chromatography

Submitted on March 1, 1966
Accepted on October 3, 1966


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