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Journal of Lipid Research, Vol. 8, 152-154, March 1967
Copyright © 1967 by Lipid Research, Inc.

Separation of 24- and 25-dehydrocholesterols; and the impure state of commercial desmosterol preparations

James A. Svoboda and Malcolm J. Thompson

Insect Physiology Laboratory, Entomology Research Division, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705

Chromatographic methods for the separation of 24- and 25-dehydrocholesterols are described. The purities of three commercial samples of 24-dehydrocholesterol examined by thin-layer and gas-liquid chromatography were only 42, 79, and 80%, respectively; a commercial sample of radioactive 24-dehydrocholesterol was shown to contain 40% 25-dehydrocholesterol.

Supplementary key words 24- and 25-dehydrocholesterols • separation • silver nitrate • thin-layer • column • chromatography • radiochemical purity

Submitted on September 9, 1966
Accepted on October 27, 1966


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