Journal of Lipid Research, Vol. 8, 191-195, May 1967
Copyright © 1967 by Lipid Research, Inc.
Acetate-1-14C incorporation into polyunsaturated fatty acids of phospholipids of developing chick brain
Kanji Miyamoto , Leo M. Stephanides , and Joseph Bernsohn
Neuropsychiatric Research Laboratory, Veterans Administration Hospital, Hines, Illinois 60141, and Northwestern University Medical School, Chicago, Illinois 60611
The incorporation of acetate-1-14C into the polyunsaturated fatty acids of glycerophosphatides of chick embryonic brain has been studied. After the injection of acetate-1-14C into the yolk sac, differences were found in the degree of labeling of the major fatty acids of the 
3 and 6 series. Arachidonic acid (20:46) showed a high degree of radioactivity while docosahexaenoic acid (22:63) was poorly labeled, at a period of brain development when both fatty acids were being actively deposited. Evidence is presented to indicate that the low activity in docosahexaenoic acid is not explicable on the basis of either a low or high rate of turnover of this polyenoic acid. Similar results were obtained whether the rapid early or slower late stage of brain development was examined. It is suggested that the elongation of 3 and 6 series acids may be under the control of different regulatory mechanisms.
Supplementary key words polyunsaturated fatty acids • 3 and 6 • biosynthesis • acetate • developing chick brain • arachidonic acid • docosahexaenoic acid • glycerophosphatides
Submitted on October 10, 1966
Revised on December 1, 1966
Accepted on January 9, 1967
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