Journal of Lipid Research, Vol. 8, 281-282, May 1967
Copyright © 1967 by Lipid Research, Inc.
Removal of the sphingolipid impurity from preparations of yeast phosphatidyl inositol
William E. Trevelyan
Distillers Company (Yeast) Ltd., Great Burgh, Epsom, Surrey, England
A complex sphingolipid containing inositol and mannose, present in lipid extracted from toluene-autolyzed baker's yeast, was eluted from silicic acid columns immediately after phosphatidyl inositol, and was the main nitrogenous impurity in crude preparations of this phospholipid. Nitrogenfree phosphatidyl inositol was obtained by rechromatography on alumina. Modifications to the chromatographic procedure also gave diphosphatidyl glycerol containing the theoretical 4.29% P.
Supplementary key words yeast phosphatidyl inositol sphingolipid silicic acid chromatography alumina chromatography diphosphatidyl glycerol
Submitted on November 18, 1966
Accepted on January 3, 1967