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Journal of Lipid Research, Vol. 8, 282-284, May 1967
Copyright © 1967 by Lipid Research, Inc.

Thin-layer chromatography of sterols on neutral alumina impregnated with silver nitrate

R. Kammereck , Wen-hui Lee , A. Paliokas , and G. J. Schroepfer Jr.

Division of Biochemistry and Department of Chemistry and Chemical Engineering, University of Illinois, Urbana, Illinois 61803

Thin-layer chromatography for the rapid separation of several sterols on neutral alumina impregnated with silver nitrate is described. The method is particularly effective for sterols that differ in the number and location of olefinic bonds.

Supplementary key words thin-layer chromatography • sterols • alumina-silver nitrate • intermediates • cholesterol biosynthesis

Submitted on July 19, 1966
Revised on January 3, 1967
Accepted on January 6, 1967


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