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Journal of Lipid Research, Vol. 8, 285-287, May 1967
Copyright © 1967 by Lipid Research, Inc.

Comparison of two methods for the separation of polar from nonpolar lipids

L. Hartman

Food Chemistry Division, Department of Scientific and Industrial Research, Wellington, New Zealand

The countercurrent extraction method of Galanos and Kapoulas for the separation of phospholipids from triglycerides was compared with dialysis in petroleum ether in an apparatus modified from that of Eberhagen and Betzing. The efficiencies of the methods are similar, but dialysis, which is slower, can yield triglycerides that are entirely free from phosphorus. The behavior of free fatty acids and monoglycerides is also described.

Supplementary key words countercurrent extraction • dialysis • phospholipids • triglycerides • polar • nonpolar • lipids • free fatty acids • monoglycerides

Submitted on June 6, 1966
Revised on January 17, 1967
Accepted on January 19, 1967


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