J. Lipid Res.
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Journal of Lipid Research, Vol. 8, 295-299, July 1967
Copyright © 1967 by Lipid Research, Inc.

Separation of neutral glycosphingolipids and sulfatides by thin-layer chromatography

Vladimir P. Skipski , Anson F. Smolowe , and Marion Barclay

Division of Experimental Chemotherapy, Sloan-Kettering Institute for Cancer Research, New York 10021, and Sloan-Kettering Division, Graduate School of Medical Sciences, Cornell University Medical College, New York 10021

Two one-dimensional systems for separation of glycolipids from total lipid extracts of tissues by thin-layer chromatography are described. System I used, as adsorbent, an alkaline mixture of silica gel without CaSO4 binder (75%) and magnesium silicate (25%), and the lipids were "developed" with three successive solvent mixtures. The separated compounds (from the fastest to the slowest moving) were: ceramide, ceramide monohexosides, sulfatides, ceramide dihexosides, psychosine, ceramide trihexosides, and ceramide N-acetylhexosamine trihexosides.

In system II a two-step development was used on an adsorbent consisting of silica gel without CaSO4 binder (80%) and magnesium silicate (20%). The separated compounds were: ceramides, ceramide monohexosides, and ceramide dihexosides. Psychosine and sulfatides as well as ceramide trihexosides and ceramide N-acetylhexosamine trihexosides were not separated.

In both systems all neutral lipids moved to the very top of the chromatogram and phospholipids stayed at the origin. Application of systems I and II for separation of glycolipids was demonstrated on total lipid extracts from animal tissues.

Supplementary key words glycolipids • neutral glycolipids • glycosphingolipids • aminoglycolipids • separation • ceramide monohexosides • ceramide dihexosides • ceramide trihexosides • psychosine • sulfatides • ceramides • cerebrosides • ceramide N-acetylhexosamine trihexosides • magnesium silicate • thin-layer chromatography

Submitted on November 7, 1966
Accepted on February 20, 1967


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