Journal of Lipid Research, Vol. 8, 335-341, July 1967
Copyright © 1967 by Lipid Research, Inc.
Incorporation of acetate into fatty acids and lecithin by lung slices from fetal and newborn lambs
Nobukazu Chida and Forrest H. Adams
Division of Cardiology, Department of Pediatrics, University of California at Los Angeles School of Medicine, Los Angeles, California 90024
Incorporation of acetate-1-14C into phospholipids and fatty acids by lung slices from fetal and newborn lambs and from ewes was studied in vitro. The distribution of radioactivity in the fatty acids of neutral lipids, phospholipids, and lecithin was determined. Acetate-1-14C was incorporated into myristic, palmitic, and C18 fatty acids. Of the lecithin fatty acids, myristic and palmitic were the major radioactive fatty acids.
The results indicate that the lung of fetal lambs is able to synthesize lecithin containing saturated fatty acids, a major constituent of pulmonary surfactant. A marked increase in the rate of acetate incorporation into lecithin was observed during maturation, and these rates were higher than those obtained in the ewes. A possible relationship between developmental changes in lecithin biosynthesis and pulmonary surfactant is discussed.
Supplementary key words incorporation • acetate-1-14C • lecithin • saturated • fatty acids • lung • sheep • fetus • maturation • newborn • pulmonary surfactant
Submitted on September 8, 1966
Accepted on March 17, 1967
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