Journal of Lipid Research, Vol. 8, 352-358, July 1967
Copyright © 1967 by Lipid Research, Inc.
Direct gas chromatographic examination of total lipid extracts
A. Kuksis , L. Marai , and D. A. Gornall
Banting and Best Department of Medical Research, University of Toronto, Toronto, Canada
The conditions for gas-liquid chromatography described in a previous publication for the improved separation of natural triglycerides have been further modified to permit a direct examination of total lipid extracts. For this purpose the range of temperature programming has been extended to 100-350°C. Under optimum conditions, complete separations and quantitative estimates are obtained for free fatty acids, free sterols, diglycerides, steryl esters, and triglycerides. The separations are based on differences in molecular weights. Glycero-phosphatides present in the sample seem to be pyrolyzed to compounds with retention times similar to those of diglycerides.
The accuracy of the method is examined for standard mixtures of neutral lipids and fatty acids, and applications are illustrated with samples of the total plasma lipids of man. The lipid compositions obtained are compared with those data derived by conventional analyses. Although the method is rapid, its successful application to quantitative routine analyses requires extreme care.
Supplementary key words gas-liquid chromatography • high-temperature programming • on-column injector • dual column operation • total lipid extracts • pyrolysis of lecithins • plasma lipids
Submitted on January 6, 1967
Accepted on April 7, 1967
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