Journal of Lipid Research, Vol. 8, 391-395, July 1967
Copyright © 1967 by Lipid Research, Inc.

Improved reagent for trimethylsilylation of sphingolipid bases

H. E. Carter and R. C. Gaver

Division of Biochemistry, Noyes Laboratory of Chemistry, University of Illinois, Urbana, Illinois 61801

This paper describes the trimethylsilylation of sphingolipid bases under conditions that give derivatives of improved stability. The retention times of the common C₁₈ and C₂₀ long-chain bases, including the anhydro bases, obtained on a commercially available gas-liquid chromatographic column with a nonpolar stationary phase are given. Data are also presented on the separation of the erythro and threo isomers of sphingosine and dihydrosphingosine, as the trimethylsilyl ethers of the N-acetyl derivatives.

Supplementary key words sphingolipid bases • gas-liquid chromatography • trimethylsilyl ether derivatives • separation • long-chain bases • erythro and threo isomers • trimethylsilylation • N-acetyl bases • C₁₈- and C₂₀-sphingosine, dihydrosphingosine, and phytosphingosine • dehydrophytosphingosine • anhydro-C₁₈-phytosphingosine • anhydro-C₁₈-dehydrophytosphingosine

Submitted on January 23, 1967
Accepted on April 10, 1967

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