Journal of Lipid Research, Vol. 8, 406-408, July 1967
Copyright © 1967 by Lipid Research, Inc.

1,2-³H-Cholesterol as a tracer in studies of human cholesterol metabolism

Peter D. S. Wood , Daphne Myers , Yuen-Ling Lee , Ryuzo Shioda , and Laurance W. Kinsell

Institute for Metabolic Research, Highland General Hospital, Oakland, California 94606

1,2-³H-Cholesterol was fed to a subject together with 4-¹⁴C-cholesterol at a known ³H/¹⁴C ratio. The ratio was satisfactorily preserved in cholesterol recovered from plasma, red cells, and bile, and in bile acids of bile.

Isotopic fractionation was seen during thin-layer chromatographic isolation of cholesterol. In work with 1,2-³H-cholesterol or its metabolites care should be taken to recover chromatographic bands in their entirety.

Supplementary key words cholesterol • 1,2-³H-cholesterol • 4-¹⁴C-cholesterol • thin-layer chromatography • isotope effect • plasma • red blood cells • bile acids • man

Submitted on December 12, 1966
Accepted on March 15, 1967

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