Journal of Lipid Research, Vol. 8, 494-500, September 1967
Copyright © 1967 by Lipid Research, Inc.

Characterization and identification of glyceryl ether diesters present in tumor cells

Randall Wood and Fred Snyder

Medical Division, Oak Ridge Institute of Nuclear Studies, Oak Ridge, Tennessee 37830

The previously unidentified neutral lipid present in tumor tissues has been isolated from Ehrlich ascites cells and unequivocally identified as a lipid class of glyceryl ether diesters containing various degrees of unsaturation, and ranging in approximate molecular weight from 760 to 990. The glyceryl ether diester fraction was shown to be free from neutral plasmalogens (glyceryl diacyl alk-1'-enyl ethers).

The tumor lipid was subjected to saponification, transesterification, and lithium aluminum hydride reduction. The glyceryl monoethers that resulted from deacylation were the 1-isomers ranging in hydrocarbon chain length from C₁₂ to C₂₄. The predominant glyceryl ethers were the hexadecyl (49%), octadecyl (21%), and octadecenyl (14%) derivatives. Saturated and mono- and polyunsaturated fatty acids ranging in chain length from C₁₂ to C₂₄ carbon atoms were esterified to the glyceryl monoether.

Gas-liquid chromatography, thin-layer chromatography, and nuclear magnetic resonance and infrared spectroscopy were used to characterize and identify the intact tumor lipid and its derived products.

Supplementary key words glyceryl ether diesters • glyceryl monoethers • tumor lipids • gas-liquid chromatography • IR spectroscopy • nuclear magnetic resonance • thin-layer chromatography

Submitted on March 20, 1967
Accepted on May 22, 1967

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