Journal of Lipid Research, Vol. 8, 501-507, September 1967
Copyright © 1967 by Lipid Research, Inc.

Separation and size determination of human serum lipoproteins by agarose gel filtration

Simeon Margolis

Departments of Medicine and Physiological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

A method is described for the separation of the three major classes of human serum lipoproteins by gel filtration on columns of 4 and 6% agarose gel. After calibration of the columns, the elution volumes of the lipoproteins were used to calculate the molecular sizes and molecular weights of these macromolecules. The technique was employed to demonstrate aggregation of low density lipoprotein following partial delipidation, partial proteolysis, or mild heat denaturation. Agarose gel filtration shows promise as a useful method for the isolation, purification, and characterization of lipoproteins.

Supplementary key words gel filtration • serum • lipoproteins • agarose • separation • molecular weight • molecular size • aggregation • delipidation • denaturation • proteolysis

Submitted on March 10, 1967
Accepted on May 22, 1967

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